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	Provedor de dados BJM (16) BABT (10) Electron. J. Biotechnol. (7) Arq. Bras. Med. Vet. Zootec. (3) Ciência Rural (3) R. Bras. Zootec. (2) Rev. Bras. Zool. (1) OAK (1) Biological Sciences (1) Anais da ABC (AABC) (1) ArchiMer (1) BJMBR (1) Ciência e Agrotecnologia (1) Genet. Mol. Biol. (1) PAB (1) Rev. Bras. Ciênc. Avic. (1) Mais... Autor Almeida,Ana Paula Gottlieb (2) Arulpandi,I. (2) Baldisserotto,Bernardo (2) Freire,Denise Maria Guimarães (2) Freitas,Sidinéa Cordeiro de (2) Geetha,A. (2) Sangeetha,R. (2) Aggelis,George (1) Alberto,Thiago Grando (1) Alegre,Ranulfo Monte (1) Alves,Maria Helena (1) Aquino,A.A. (1) Araújo,Maria Elisa Melo Branco de (1) Aroca Arcaya,Germán (1) Augusto,L.F.B. (1) Bachmann,Luciano (1) Baião,N.C. (1) Banerjee,Rintu (1) Behr,Everton Rodolfo (1) Benedetti,Silvia (1) Mais... Palavra-chave Lipase (51) Protease (7) Amylase (5) Esterification (4) Immobilization (3) Optimization (3) Trypsin (3) Aspergillus niger (2) Characterization (2) Hydrolysis (2) Mutation (2) Pepsin (2) Pseudomonas (2) Purification (2) Solid-state fermentation (2) Yarrowia lipolytica (2) Agglutinin (1) Agricultural residues (1) Agroindustrial residues (1) Albumin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (42) Journal article (7) Text (1) Ano 2020 (2) 2018 (1) 2017 (3) 2016 (5) 2015 (3) 2014 (5) 2013 (6) 2012 (1) 2011 (2) 2010 (2) 2009 (4) 2008 (2) 2007 (3) 2006 (5) 2005 (2) 2004 (1) 2002 (2) 2001 (1) 2000 (1) Mais... País Brazil (42) Chile (7) France (1) Japan (1) Idioma Inglês (43) Português (7) Japonês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 51 Primeira ... 1 2 3 ... Última Composition of gastrointestinal content, protease and lipase activities in summer and winter of four freshwater siluriforms (Teleostei: Actinopterygii) with two different feeding habits Rev. Bras. Zool. Almeida,Ana Paula Gottlieb; Zardo,Everton Luis; Toni,Candida; Behr,Everton Rodolfo; Silva,Leila Picolli da; Vieira,João Paes; Loro,Vania Lúcia; Baldisserotto,Bernardo. ABSTRACT The aim of this study was to determine the composition of gastrointestinal content and protease and lipase activities in summer and winter as well as to evaluate the relationship between digestive enzyme activity and centesimal composition of gastrointestinal content and feeding habits of two omnivorous species, Rhamdia quelen (Quoy & Gaimard, 1824) and Pimelodus maculatus (LaCèpede, 1803), and of two detritivorous species, Loricariichthys anus (Valenciennes, 1835) and Hypostomus commersoni (Valenciennes, 1836). The activities of pepsin, trypsin, chymotrypsin, and lipase, and the levels of proteins and lipids in the gastrointestinal tract, were evaluated. The enzyme activities were not related to the centesimal composition of gastrointestinal... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chymotrypsin; Fish; Lipase; Pepsin; Trypsin. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100322 Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease BJM Alves,Maria Helena; Campos-Takaki,Galba M.; Porto,Ana Lúcia Figueiredo; Milanez,Adauto Ivo. Fungi are well known by their ability to excrete enzymes into the environment. Among them, representatives of Mucor Fresen. have important biotechnological potential and some of them produce industrial enzymes. This work studied amylase, lipase, polygalacturonase and protease production by fifty-six isolates of Mucor belonging to 11 different taxa, selected from herbivores dung using solid media. The results showed that the majority of the isolates presented several enzymatic activities with predominance of polygalacturonase (96%), followed by amylase (84%), protease (82%) and lipase (66%). Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungal enzymes; Mucor; Amylase; Lipase; Polygalacturonase; Protease; Herbivorous dung. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400009 Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase BJM Araújo,Maria Elisa Melo Branco de; Campos,Paula Renata Bueno; Alberto,Thiago Grando; Contesini,Fabiano Jares; Carvalho,Patrícia de Oliveira. Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4 °C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Immobilization; Soybean oil; Polyunsaturated fatty acids. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401006 Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia Electron. J. Biotechnol. Caballero Valdés,Eduardo; Wilson Soto,Lorena; Aroca Arcaya,Germán. The preparation of cross-linked enzyme aggregates (CLEAs) of lipase has been a challenge due the low amount of lysine residues that lipases have on their surface. The results show that CLEAs prepared using dextran aldehyde (100-200KDa) have a higher hydrolysis activity and particle size (activities between 3186 ± 21 U/g of CLEA and 4800 ± 30 U/g of CLEA and particle sizes between 52.6 ± 18.7 µm and 126.2 ± 53.5 µm) than CLEAs prepared with glutaraldehyde (0.1 KDa) (activities between 894 ± 16 U/g of CLEA and 2874 ± 20 U/g of CLEA and particle sizes between 21.2 ± 5.1 µm and 83.4 ± 24.9 µm); Thermal stability assays of bioctalysts at 60ºC at pH 7.0 using phosphate buffer 25 mM showed that CLEAs prepared with dextran aldehyde have lower residual... Tipo: Journal article Palavras-chave: Albumin; Dextran aldehyde; Glutaraldehyde; Immobilisation; Lipase. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300010 Efeitos do período de jejum entre o nascimento e o alojamento de pintos de corte e da adição de óleo à ração sobre o desenvolvimento do trato gastrintestinal e concentração de lipase Arq. Bras. Med. Vet. Zootec. Cançado,S.V.; Baião,N.C.. Os objetivos deste trabalho foram determinar o efeito de três períodos de jejum (zero, 24 e 48 horas) entre o nascimento e o alojamento de pintos e da adição de óleo à ração sobre o crescimento alométrico dos órgãos digestivos e estudar a atividade da lipase no pâncreas e intestino delgado. Foram utilizados 720 pintos de corte machos, criados de um a 15 dias de alojamento. As avaliações do crescimento dos órgãos digestivos foram feitas nos dias zero, três, seis, nove, 12 e 15 de idade. As avaliações da concentração de lipase no pâncreas foram feitas nos dias zero, três, seis, nove, 12 e 15, e no intestino delgado nos dias zero, três e seis. O delineamento experimental foi em arranjo fatorial 3 x 2 (três períodos de jejum e dois tipos de ração). O... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pintos de corte; Trato gastrintestinal; Lipase; Jejum. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000600011 Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles BABT Carneiro,Lara Aparecida Buffoni Campos; Costa-Silva,Tales Alexandre; Souza,Cláudia Regina Fernandes; Bachmann,Luciano; Oliveira,Wanderley Pereira; Said,Suraia. This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated CAM showed better performance retaining 88.4% of the enzymatic activity, with 68.2% of immobilization efficiency (IE). The immobilized enzyme retained an activity of about 53.5 % after five reuses, using p-NPP as substrate. Physical adsorption of lipase onto highly deacetylated CHM showed 46.2 % of enzymatic activity and 28.6% of IE. This immobilized derivative did not lose activity up to 80 days of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme immobilization; Chitosan; Cercospora kikuchii; Lipase. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400578 Comparative esterification of phenylpropanoids versus hydrophenylpropanoids acids catalyzed by lipase in organic solvent media Electron. J. Biotechnol. Cassani,Julia; Luna,Héctor; Navarro,Arturo; Castillo,Edmundo. The esterification of phenylpropanoid and hydrophenylpropanoid acids, catalyzed by candida antarctica lipase B (CAL-B), with several alcohols has demonstrated that the substitution pattern on the aromatic ring has a very significant influence on the reactivity of the carboxyl group due, mainly, to electronic effects, when compared to the unsaturated acids with the hydrogenated acids. It is also clear that in the saturated acids there still remain some unclear effects related to the aromatic substituents. Tipo: Journal article Palavras-chave: CAL-B; Electronic effects; Esterification; Lipase; Phenylpropanoid acids. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400004 Lipase production by solid-state fermentation in fixed-bed bioreactors BABT Cavalcanti,Elisa d'Avila Costa; Gutarra,Melissa Limoeiro Estrada; Freire,Denise Maria Guimarães; Castilho,Leda dos Reis; Sant'Anna Júnior,Geraldo Lippel. In the present work, packed bed bioreactors were employed with the aim of increasing productivity and scaling up of lipase production using Penicillium simplicissimum in solid-state fermentation. The influence of temperature and air flow rate on enzyme production was evaluated employing statistical experimental design, and an empirical model was adjusted to the experimental data. It was shown that higher lipase activities could be achieved at lower temperatures and higher air flow rates. The maximum lipase activity (26.4 U/g) was obtained at the temperature of 27°C and air flow rate of 0.8 L/min. Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-state fermentation; Fixed-bed bioreactors; Lipase; Penicillium simplicissimum. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400010 Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi BJM Colla,Luciane Maria; Primaz,Andreiza Lazzarotto; Benedetti,Silvia; Loss,Raquel Aparecida; Lima,Marieli de; Reinehr,Christian Oliveira; Bertolin,Telma Elita; Costa,Jorge Alberto Vieira. Abstract A Plackett–Burman Factorial Design of 16 experiments was conducted to assess the influence of nine factors on the production of lipases by filamentous fungi. The factors investigated were bran type (used as the main carbon source), nitrogen source, nitrogen source concentration, inducer, inducer concentration, fungal strain (Aspergillus niger or Aspergillus flavus were selected as good lipase producers via submerged fermentation), pH and agitation. The concentration of the yeast extract and soybean oil and the pH had a significant effect (p < 0.05) on lipase production and were consecutively studied through a Full Factorial Design 23, with the concentration of yeast extract and pH being significant (p < 0.05). These variables were optimized... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural residues; Lipase; Optimization; Submerged bioprocess. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200461 Hydrolytic potential of a psychrotrophic Pseudomonas isolated from refrigerated raw milk BJM Corrêa,Ana Paula F.; Daroit,Daniel J.; Velho,Renata V.; Brandelli,Adriano. The production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a Pseudomonas sp. belonging to the Pseudomonas jenssenii group, was studied. This bacterium produced proteolytic and lipolytic enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). High levels of α-glucosidase were produced in skim milk broth. Hydrolytic enzymes detected in skim milk broth are of particular concern, indicating that these enzymes could be produced by Pseudomonas sp. during the cold storage of raw milk, contributing to the spoilage problem in milk and dairy products. Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas; Hydrolases; Protease; Lipase; Glycosidase. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400034 Utilization of agroindustrial residues for lipase production by solid-state fermentation BJM Damaso,Mônica Caramez Triches; Passianoto,Moisés Augusto; Freitas,Sidinéa Cordeiro de; Freire,Denise Maria Guimarães; Lago,Regina Celi Araujo; Couri,Sonia. The aim of this work was to produce lipases by solid-state fermentation (SSF) using, as substrate, agroindustrial residue supplemented with by-products from corn oil refining process or olive oil. For a group of ten fungi strains selected in the first steps, the lipase activity obtained by SSF varied from 7.7 to 58.6 U/g of dry substrate (gds). Among the evaluated strains, the Aspergillus niger mutant 11T53A14 was selected by presenting the best enzymatic production. For the fermentation tests, two substrates were also investigated: wheat bran and corn cob, both supplemented with olive oil. The best results were obtained with wheat bran. Additionally, three industrial by-products from corn oil refining (soapstock, stearin and fatty acids) were evaluated as... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Solid state fermentation; Agroindustrial residues; Corn oil by-products. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400015 Enhanced production of Aspergillus tamarii lipase for recovery of fat from tannery fleshings BJM Dayanandan,A.; Rani,S. Hilda Vimala; Shanmugavel,M.; Gnanamani,A.; Rajakumar,G. Suseela. The influence of various oil cakes has been investigated for high level production of lipase using Aspergillus tamarii MTCC 5152. By solid state fermentation in wheat bran containing 2.5% w/w gingili oil cake at 70% v/w moisture content the fungus produced a maximal yield of lipase (758 ± 3.61 u/g) after 5 days of incubation using 2% v/w inoculum containing 10(6) spores/mL. Wheat bran and gingili oil cake with supplementation of gingili oil (1.0% w/w), glucose (0.5% w/w) and peptone (0.5% w/w) gives an increased enzyme production of 793 ± 6.56 u/g. The enzyme shows maximum activity at pH 7.0, temperature 50 °C and was stable between the pH 5.0-8.0 and temperature up to 60 °C. Crude lipase (3%) applied to tannery fleshing shows 92% fat solubility. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat bran; Oil cakes; Lipase; Tannery fleshing; Fat hydrolysis. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400010 Ferulic acid lowers body weight and visceral fat accumulation via modulation of enzymatic, hormonal and inflammatory changes in a mouse model of high-fat diet-induced obesity BJMBR de Melo,T.S.; Lima,P.R.; Carvalho,K.M.M.B.; Fontenele,T.M.; Solon,F.R.N.; Tomé,A.R.; de Lemos,T.L.G.; da Cruz Fonseca,S.G.; Santos,F.A.; Rao,V.S.; de Queiroz,M.G.R.. Previous studies have reported on the glucose and lipid-lowering effects of ferulic acid (FA) but its anti-obesity potential has not yet been firmly established. This study investigated the possible anti-obesitogenic effects of FA in mice fed a high-fat diet (HFD) for 15 weeks. To assess the antiobesity potential of FA, 32 male Swiss mice, weighing 20–25 g (n=6–8 per group) were fed a normal diet (ND) or HFD, treated orally or not with either FA (10 mg/kg) or sibutramine (10 mg/kg) for 15 weeks and at the end of this period, the body weights of animals, visceral fat accumulation, plasma levels of glucose and insulin hormone, amylase and lipase activities, the satiety hormones ghrelin and leptin, and tumor necrosis factor-α (TNF-α) and monocyte... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ferulic acid; Anti-obesity; High-fat diet; Leptin; Lipase; Tumor necrosis factor-α. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100603 Enzymatic comparison and mortality of Beauveria bassiana against cabbage caterpillar Pieris brassicae LINN BJM Dhawan,Manish; Joshi,Neelam. Abstract Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (109 conidia/ml), and the minimum mortality (30.00%) was recorded in B. bassiana MTCC 6291 at a lower concentration (107 conidia/ml) after ten days of treatment. The extracellular cuticle-degrading enzyme activities of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Entomopathogenic fungi; Lipase; Mortality; Protease. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300522 Reatividade in vitro de lipase submetida a diferentes tratamentos tecnológicos Arq. Bras. Med. Vet. Zootec. Ferreira,V.P.A.; Ferreira,W.M.; E.O.S.,Saliba; Teixeira,A.O.. Analisou-se a atividade enzimática in vitro de uma lipase em diferentes condições de temperatura (simulando o processo de peletização) e pH (acidez gástrica) e na presença de íons minerais e metálicos. Para avaliar a estabilidade térmica, a enzima foi incubada a 80(0)C por quatro tempos (0, 5, 10 e 15 minutos). O efeito do pH foi estudado submetendo-se a enzima a quatro valores de pH (7,3, 2,0, 3,2 e 5,0). Para avaliar o efeito da lipase na presença de íons minerais e metálicos, incubou-se a enzima em diferentes soluções contendo cálcio, cobre, cobalto, manganês e ferro. Frente às diferentes condições, a lipase conservou atividade parcial, mas não pôde ser considerada uma enzima ideal em função do efeito inibidor promovido pelo pH ácido e pelas soluções... Tipo: Info:eu-repo/semantics/article Palavras-chave: Atividade; Lipase; Íons; PH; Temperatura. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300011 Screening of microbial lipases and evalutaion of their potential to produce glycerides with high gamma linolenic acid concentration BJM Fregolente,Patricia B.L.; Fregolente,Leonardo V.; Maciel,Maria R.W.; Carvalho,Patricia O.. Gamma-linolenic acid (GLA, 18:3, cis- 6,9,12-octadecatrienoic acid), an important compound in n-6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids). Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil, strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a special... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma linolenic acid; Borage oil; Lipase; Geotrichum candidum. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400004 Virulence-associated characteristics of Enterococcus faecalis strains isolated from clinical sources BJM Furumura,Márcia T.; Figueiredo,Patricia M.S.; Carbonell,Gleize V.; Darini,Ana Lucia da Costa; Yano,Tomomasa. Thirty-two clinical isolates of Enterococcus faecalis were screened for virulence factors. Twenty-four (75%) isolates produced hemolysin on Mueller-Hinton blood agar plates with sheep erythrocytes. However, the cell free heat-stable hemolysin was detected in all isolates (100%) of E. faecalis when grown in BHI-GA (BHI medium supplemented with 1% glucose and 0.03% L-arginine), but not in BHI broth alone. Twenty-four isolates (75%) produced caseinase and 23 (71.9%) lipase, but none of the isolates produced gelatinase. Fifteen (46.9%) culture filtrates caused rounding and membrane alterations with blebbing formation followed by death in HeLa and HEp-2 cells, but not in Vero cells. Thirteen isolates (40.6%) agglutinated rabbit erythrocytes, but did not produce... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus faecalis; Virulence factors; Hemolysin; Proteases; Lipase; Cytotoxin; Biofilm. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300007 Efeito da suplementação enzimática em rações com farelo de soja e soja integral extrusada sobre a digestibilidade de nutrientes, o fluxo de nutrientes na digesta ileal e o desempenho de frangos R. Bras. Zootec. Garcia,Elis Regina de Moraes; Murakami,Alice Eiko; Branco,Antonio Ferriani; Furlan,Antonio Claudio; Moreira,Ivan. Três experimentos foram realizados com o objetivo de avaliar o efeito da suplementação enzimática em rações à base de milho e soja sobre o desempenho (Experimento 1), a digestibilidade de nutrientes (Experimento 2) e o desempenho e o fluxo de nutrientes na digesta ileal (Experimento 3) em frangos de corte. No Experimento 1, para o cálculo das rações experimentais, a composição química do farelo de soja (FS) e da soja integral extrusada (SIE) foi superestimada em 7% para os valores de EM e PB e 5% para Met, Met+Cis e Lis (Trat. C, D e E) e 9% para EM, 7% para PB e 5% para Met, Met+Cis e Lis (Trat. F, G e H), quando houve a adição de enzimas (Allzyme Vegpro<FONT FACE=Symbol>â</FONT> e Allzyme Lipase<FONT FACE=Symbol>â</FONT>).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Complexo multienzimático; Farelo de soja; Frango de corte; Lipase; Soja integral extrusada. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982000000500020 Effect of methyl branching of C8H18 alkanes and water activity on lipase-catalyzed enantioselective esterification of ibuprofen Electron. J. Biotechnol. Gottemukkala,Vijay V; Saripella,Kalyan K; Kadari,Anil K; Neau,Steven H. The purpose of this research was to study the effect of the methyl branching of a high log P alkane solvent and the water activity in the organic medium on the initial rate and the enantioselectivity of ibuprofen esterification catalyzed by Candida rugosa lipase. Resolution of ibuprofen is important because S-(+)-ibuprofen has the desired pharmacological activity, whereas the R-(-)-enantiomer causes much of the side effects. The Candida rugosa lipase-catalyzed reaction in isooctane at 40ºC and 0.73 water activity gave the best results, both in terms of the initial reaction rate and the enantioselectivity of the reaction. An increase in water activity allowed a higher reaction rate and enantiomeric excess in each of the four solvents. An increase in methyl... Tipo: Journal article Palavras-chave: Enantioselective; Esterification; Ibuprofen; Lipase; Solvent-effect; Water activity. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100002 Use of Plackett-Burman design for rapid screening of nitrogen and carbon sources for the production of lipase in solid state fermentation by Yarrowia lipolytica from mustard oil cake (Brassica napus) BJM Imandi,Sarat Babu; Karanam,Sita Kumari; Garapati,Hanumantha Rao. Mustard oil cake (Brassica napus), the residue obtained after extraction of mustard oil from mustard oil seeds, was investigated for the production of lipase under solid state fermentation (SSF) using the marine yeast Yarrowia lipolytica NCIM 3589. Process parameters such as incubation time, biomass concentration, initial moisture content, carbon source concentration and nitrogen source concentration of the medium were optimized. Screening of ten nitrogen and five carbon sources has been accomplished with the help of Plackett-Burman design. The highest lipase activity of 57.89 units per gram of dry fermented substrate (U/gds) was observed with the substrate of mustard oil cake in four days of fermentation. Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Mustard oil cake; Plackett-Burman design; Yarrowia lipolytica. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300041 Registros recuperados: 51 Primeira ... 1 2 3 ... Última

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